Strong background in bone diseases, virology, neurosciences, nephrology
Holder of a Ph.D in Biochemistry, Cellular and Molecular Biology, I have been focused on the preclinical development of therapeutic molecules, the elucidation of the mechanisms of action of these molecules and the discovery of the mechanisms involved in human pathologies (osteoarthritis, genetic brain and kidney diseases).
In this context, beyond technical skills, I have developed soft skills which allowed me to carry out the missions I have been entrusted :
Management skills :
Supervision of CROs
Coordination of expert networks
Team supervision
Scientific project management
Communication skills :
Reporting
Written and oral presentation of data
Redaction of scientific projects and documents
Scientific skills :
Study design
Development of procedures
Bibliographic and technological survey
Project review
The development of therapeutic molecules is still topical and remains a public health challenge in which I still want to get involved as a preclinical R&D project manager.
Cell lines culture : epithelial cells (cos, HeLa, HEK, HK-2, MDCK, IMCD, SRA 01/04, T47D), cells from human neuroblastoma SH-SY5Y
Expert
3-D cell culture, cell culture on Petri dishes, cell polarization on transwell filters.
Advanced
Cellular in vitro tests: Measurement of trans epithelial resistance, Migration assay (wound healing and transwell), Uptake assay (EGF receptor, albumin), Proliferation assay (BrDU), Adhesion assay (Vybrant), Calcium live-imaging
Expert
Cellular biology: Murine embryos extraction from uterine horns and isolation of embryonic hippocampi, transitory transfection in primary cells and in cell lines (liposome, electroporation), establishment of stable cell lines, production and viral infection
Expert
Molecular biology: DNA and RNA extraction PCR and qRT-PCR Site-directed mutagenesis Cloning and sub cloning Bacteria transformation Transcriptomic analyses
Advanced
Biochemistry: Protein extraction, western blot and digital quantification Production and purification of recombinant proteins GST Pull-Down Co-immunoprecipitation
Expert
Informatics: Images acquisition, treatment and analysis: MetaMorph (acquisition), ImageJ (treatment, analysis), Photoshop, Imaris (analysis).GraphPad (Prism), Endnote.Bio-informatics: databases (PubMed, Human protein atlas).
Expert
Cell imaging: Fixation and cellular staining (immunofluorescence), Wild-field and confocal microscopy, Videomicroscopy