Rodrick MONTJEAN, Ph.D

Strong background in bone diseases, virology, neurosciences, nephrology

Holder of a Ph.D in Biochemistry, Cellular and Molecular Biology, I have been focused on the preclinical development of therapeutic molecules, the elucidation of the mechanisms of action of these molecules and the discovery of the mechanisms involved in human pathologies (osteoarthritis, genetic brain and kidney diseases).

In this context, beyond technical skills, I have developed soft skills which allowed me to carry out the missions I have been entrusted :

Management skills :

Supervision of CROs
Coordination of expert networks
Team supervision
Scientific project management

Communication skills :

Reporting
Written and oral presentation of data
Redaction of scientific projects and documents

Scientific skills :

Study design
Development of procedures
Bibliographic and technological survey
Project review

The development of therapeutic molecules is still topical and remains a public health challenge in which I still want to get involved as a preclinical R&D project manager.

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Experiences

Missions :
Preclinical development of RNA-based antiviral molecules targeting arboviruses: from design to preclinical regulatory package
Management skills :
- Search for CROs, academic collaborators
- Contracts set up and management
- Team projects management
- Scientific project management (timelines, budget, risks)
- Quality control of the studies (GLP, GMP)
Communication skills :
- Projects reporting to the CSO, partners and collaborators
- Presentation and discussion of data at internal and international meetings
- Scientific documents redaction
- Writing of a scientific project for funds (BpiFrance-Deeptech
- Propose and write a preclinical development plan
Scientific skills :
- Identify project objectives
- From hit to lead (RNA)
- Study plans definition and design
- Design and development of GLP experimental procedures
- Analyses and interpretation of results
- Team working and troubleshooting abilities
- Literature and technologic survey

Learn more

Missions :
- Preclinical phase management for the lead candidate molecule (REG-O3) in the frame of osteoarthritis therapeutics
- Identification of the competencies needed for the clinical phase and international network of experts implementation
- Clinical trial phase design in collaboration with the Chief Scientific Officer (CSO)
- Elucidation of the mechanism of action of REG-O3 internally
Management skills :
- Search for CROs, academic collaborators and partnership with other SMEs
- Contracts set up and management
- Coordination of the international network for the writing of regulatory documents (study reports, IMPD)
- Team projects management
- Scientific project management (timelines, budget, risks)
- Quality control of the studies (GLP, GMP)
- Implementation of the quality system in the company
Communication skills :
- Projects reporting to the CSO, partners and collaborators
- Writing of a Scientific Technical Advice to the Belgian (FAMHP) and European (EMA) Health authorities
- Presentation and discussion of data at internal and international meetings
- Scientific documents redaction
- Writing of a scientific project for European funds (Eureka-Eurostars)
Scientific skills :
- Study plans definition and design
- Design and development of GLP experimental procedures
- Analyses and interpretation of results
- Team working and troubleshooting abilities
- Literature and technologic survey
- Review of scientific projects involving animals (member of the ethics committee on animal experimentation n°80)

Learn more

Mission :
- Elucidation of the genetics, molecular and cellular mechanisms of the neurological features in the frame of OCRL mutations (Lowe syndrome vs Dent disease-2)
Management skills :
- Technical team supervision (2 technicians, 3 students)
- Scientific project management (timelines, budget, risks)
- National and International collaboration (France, Germany)
- In charge of consumable (inventory management)
- In charge of a wild-field microscope
Communication skills :
- Project reporting to the supervisor
- Presentation and discussion of data internally and externally (6 poster presentations at both national/international meetings)
- Data presentation to financial support organizations (3 oral communications)
- Scientific documents redaction (4 published scientific articles, 9 abstracts and 6 posters)
- Writing of 3 scientific projects for funds (3 grants obtained via Orange Foundation, Jérôme Lejeune Foundation, French Lowe Syndrome Association)
Scientific skills :
- Design and development of GLP experimental procedures
- Analyses and interpretation of results
- Team working and troubleshooting abilities
- Literature survey
Training :
- Basis of Image J software
  Cochin Institute
  December 2012
Publications :
- Ptchd1 deficiency induces excitatory synaptic and cognitive dysfunctions in mouse.
  Ung DC, Iacono G, Méziane H, Blanchard E, Papon MA, Selten M, van Rhijn JR, Montjean R, Rucci J, Martin S, Fleet A, Birling MC, Marouillat S, Roepman R, Selloum M, Lux A, Thépault RA, Hamel P, Mittal K, Vincent JB, Dorseuil O, Stunnenberg HG, Billuart P, Nadif Kasri N, Hérault Y, Laumonnier F.
  Mol Psychiatry. 2018 May;23(5):1356-1367.
  https://www.ncbi.nlm.nih.gov/pubmed/28416808
- Functional Characterization and Rescue of a Deep Intronic Mutation in OCRL Gene Responsible for Lowe syndrome.
  Rendu J, Montjean R, Coutton C, Suri SM, Chicanne G, Petiot PA, Brocard J, Grunwald D, Rouxel FP, Payrastre B, Lunardi J, Dorseuil O, Marty I, Fauré
  Hum Mutat. 2017 Feb;38(2):152-159.
  https://www.ncbi.nlm.nih.gov/pubmed/27790796
- OCRL-mutated fibroblasts from patients with Dent-2 disease exhibit INPP5B-independent phenotypic variability relatively to Lowe syndrome cells.
  Montjean R, Aoidi R, Desbois P, Rucci J, Trichet M, Salomon R, Rendu J, Fauré J, Lunardi J, Gacon G, Billuart P, Dorseuil O.
  Hum Mol Genet. 2015 Feb 15;24(4):994-1006.
  http://www.ncbi.nlm.nih.gov/pubmed/25305077
- X-exome sequencing of 405 unresolved families identifies seven novel intellectual disability genes.
  Hu H, Haas SA, Chelly J, Van Esch H, Raynaud M, de Brouwer AP, Weinert S, Froyen G, Frints SG, Laumonnier F, Zemojtel T, Love MI, Richard H, Emde AK, Bienek M, Jensen C, Hambrock M, Fischer U, Langnick C, Feldkamp M, Wissink-Lindhout W, Lebrun N, Castelnau L, Rucci J, Montjean R, Dorseuil O, Billuart P, Stuhlmann T, Shaw M, Corbett MA, Gardner A, Willis-Owen S, Tan C, Friend KL, Belet S, van Roozendaal KE, Jimenez-Pocquet M, Moizard MP, Ronce N, Sun R, O'Keeffe S, Chenna R, van Bömmel A, Göke J, Hackett A, Field M, Christie L, Boyle J, Haan E, Nelson J, Turner G, Baynam G, Gillessen-Kaesbach G, Müller U, Steinberger D, Budny B, Badura-Stronka M, Latos-Bieleńska A, Ousager LB, Wieacker P, Rodríguez Criado G, Bondeson ML, Annerén G, Dufke A, Cohen M, Van Maldergem L, Vincent-Delorme C, Echenne B, Simon-Bouy B, Kleefstra T, Willemsen M, Fryns JP, Devriendt K, Ullmann R, Vingron M, Wrogemann K, Wienker TF, Tzschach A, van Bokhoven H, Gecz J, Jentsch TJ, Chen W, Ropers HH, Kalscheuer VM
  Mol Psychiatry. 2016 Jan;21(1):133-48.
  http://www.ncbi.nlm.nih.gov/pubmed/25644381
Oral communications :
- Syndrome de Lowe et maladie de Dent : étude fonctionnelle d’un gène modificateur
  Rodrick Montjean et Olivier Dorseuil
  Assemblée Générale de l’Association du Syndrome de Lowe (Paris, France. 19th march 2016).
- Syndrome de Lowe et maladie de Dent: Analyses moléculaires et cellulaires comparatives de fibroblastes de patients.
  Rodrick Montjean et Olivier Dorseuil
  Assemblée Générale de l’Association du Syndrome de Lowe (Paris, France. 3rd may 2014).
- Syndrome de Lowe : Conséquences sur les cellules du système nerveux (Analyse de la morphologie neuronale)
  Rodrick Montjean et Olivier Dorseuil
  Assemblée Générale de l’Association du Syndrome de Lowe (Paris, France. 6th april 2013)
Poster communications :
- Characterization of Neuronal Morphology Associated with Inactivation of Intellectual Disability and Autism Spectrum Disorders Genes.
  R. Montjean, F. Paul, J. Rucci, M. Ramos, J. Renaud, G.Pavlovic, MC. Birling, G. Iacono, H. Stunnenberg, M. Selloum, Y. Herault, J. Chelly, O. Dorseuil and P. Billuart.
  2nd International GENCODYS Conference Integrative Networks in Intellectual Disabilities (Chania, Crete, Greece. 27th-29th April 2015,).
  http://www.gencodys.eu/conference2015/conference2015.php
- Cellular and molecular mechanisms involved in intellectual deficiency of patients with Lowe syndrome
  Rodrick Montjean, Rifdat Aoidi, Pierrette Desbois, Justine Galissant, Julien Rucci, Michaël Trichet, Nicolas Lebrun, John Rendu, Julien Fauré, Rémi Salomon, Joël Lunardi, Jamel Chelly, Gérard Gacon, Pierre Billuart and Olivier Dorseuil.
  EMBO Conference-Autism and related disorders: From bench to bed (La Ciotat, France. 5th-8th September 2014).
  http://events.embo.org/14-brain/
- Compensatory mechanisms between OCRL1-mutated fibroblasts from Lowe syndrome and Dent disease patients.
  Rodrick Montjean, Rifdat Aoidi, Julien Rucci, Justine Galissant, Michael Trichet, Pierrette Desbois, Joel Lunardi, Rémi Salomon, Nicolas Lebrun, Jamel Chelly, Gérard Gacon, Pierre Billuart and Olivier Dorseuil. Congress of Young Researchers- René Descartes (Paris, France. October 2013)
  http://www.jcard.fr/index.php?page=congres
- Cognitive Disorder Genes And Neuronal Morphology Characterization
  J Rucci, R Montjean, M Ramos, J Renaud, F Laumonnier, H Stunnenberg, J Chelly, O Dorseuil, And P Billuart
  4th European Synapse Meeting (Bordeaux, France. 28th-30th August 2013)
  http://esm-bordeaux2013.risc.cnrs.fr/
- Circadian regulations of hippocampal genes and role of Ophn1, an intellectual disability gene
  J.Renaud, R. Montjean F.Dumont, S.Jacques, J.Chelly, P. Billuart and O.Dorseuil
  Gencodys 1st international conference, Integrative networks in intellectual disabilities (Paphos, Chypre. 15-17th april 2013).
  http://www.gencodys.eu/Conference/
- ID genes and synaptopathy
  J.Rucci, R.Montjean, M.Ramos, J.Renaud, J.Chelly, O.Dorseuil and P. Billuart
  Gencodys 1st international conference, Integrative networks in intellectual disabilities (Paphos, Chypre. 15-17th april 2013).
  http://www.gencodys.eu/Conference/

Mission :
- Study the normal and pathological role of nephrocystins 1 and 4 genes that are associated with nephronophthisis.
Management skills :
- Scientific project management (timelines, budget, risks)
- National and International collaboration (France, Denmark)
Communication skills :
- Project reporting to the supervisor
- Presentation and discussion of data internally and externally (4 poster presentations at international meetings)
- Scientific documents redaction (2 published scientific articles, 4 abstracts and 4 posters)
- Participation in scientific articles reviewing
Scientific skills :
- Design and development of GLP experimental procedures
- Analyses and interpretation of results
- Team working and troubleshooting abilities
- Literature survey
Publications :
- Inversin/Nephrocystin-2 is required for fibroblast polarity and directional cell migration.
  Veland IR, R Montjean, Eley L, Pedersen LB, SF Pedersen, Schwab A, Goodship J, Saunier S, ST Christensen
  PLoS One. 2013 Apr 8, 8(4).
  http://www.ncbi.nlm.nih.gov/pubmed/23593172
- INF2 mutations in Charcot-Marie-Tooth disease with glomerulopathy.
  Boyer O, Nevo F, Plaisier E Funalot B Gribouval O, Benoit G, Cong EH, Arrondel C, Head MJ, Montjean R, Richard L, Karras A, Pouteil-Noble C, Balafrej L Bonnardeaux A Canaud G Charasse C Dantal J, Deschenes G, Deteix P, Dubourg O, Petiot P, Pouthier D Leguern E, Guiochon-Mantel A, Broutin I, Gubler MC, Saunier S, Ronco P, Vallat JM, Alonso MA, Antignac C Mollet G.
  N Engl J Med. 2011 Dec 22; 365 (25):2377-88.
  http://www.ncbi.nlm.nih.gov/pubmed/22187985
Oral communication :
- Nephrocystin-1 and nephrocystin-4 regulate the Wnt pathway and are required for ciliogenesis and epithelial morphogenesis
  Helori Gaudé, Céline Burcklé, Rodrick Montjean, Flora Silbermann, Emilie Montenont, Marion Delous, Christine Vesque, Cécile Jeanpierre, Alexandre Benmerah, Philippe Bastin, Corinne Antignac, Sylvie Schneider-Maunoury and Sophie Saunier
  ASN, kidney week 2011 (Philadelphia, USA. 10th-13th november 2011).
  https://www.asn-online.org/education/kidneyweek/archives/past.aspx
Poster communications :
- Nephrocystin-1, nephrocystin-4 and nephrocystin-8 regulate Dishevelled and the Wnt pathway and are required for ciliogenesis and epithelial morphogenesis.
  Helori Gaudé, Rodrick Montjean, Flora Silbermann, Valentina Grampa, Céline Burcklé, Emilie Montenont, Marion Delous, Christine Vesque, Cécile Jeanpierre, Corinne Antignac, Fabiola Terzi ,Sylvie Schneider-Maunoury, Sophie Saunier
  Cilia 2012, 1st international scientific conference (London, UK. 17th-18th may 2012).
  http://www.eventbrite.com/e/cilia-2012-1st-international-scientific-conference-tickets-2201013290
- Nephrocystin-1 and nephrocystin-4 : role in cell migration, adhesion and Rho GTPases activation.
  Helori Gaudé, Rodrick Montjean, Flora Silbermann, Emilie Montenont, Céline Burcklé, Marion Delous, Corinne Antignac and Sophie Saunier.
  6èmes journées «Cils, flagelles, centrosomes» (Lyon, France. 15th-16th novembrer 2011).
- Loss of NPHP1 and NPHP4 in MDCK cells leads to an over activation of Rho GTPases and actin cytoskeleton reorganization
  Rodrick Montjean, Flora Silbermann, Helori-Maël Gaudé, Marion Delous, Corinne Antignac and Sophie Saunier
  5èmes journées «Cils, flagelles, centrosomes» (Dourdan, France. 20th-21th october 2010).

Mission :
- Elucidate the molecular and cellular mechanisms involved in the renal features of the Lowe syndrome.
Management skills :
- Scientific project management (timelines)
- National collaboration
- Students supervision
- In charge of cell culture reagents (testing, inventory management)
Communication skills :
- Project reporting to the supervisor
- Presentation and discussion of data internally and externally (1 poster presentation during a national meeting)
Scientific skills :
- Design and development of GLP experimental procedures
- Analyses and interpretation of results
- Team working and troubleshooting abilities
- Literature survey
Poster communication :
- Renal tubular cells of Lowe patients exhibit defective albumin uptake, associated with dysregulation of a Rac GTPase pathway and alteration of Actin cytoskeleton.
  Rodrick Montjean, Michaël Trichet, Pierrette Desbois, Rémi Salomon, Joël Lunardi, Gérard Gacon and Olivier Dorseuil.
  12th annual meeting of the «club Exocytose-Endocytose» (Presqu'île de Giens, France. 4th-6th june 2009)

Mission :
- Elucidate the molecular and cellular mechanisms involved in the renal features of the Lowe syndrome.
Management skills :
- Scientific project management (timelines)
- National collaboration
- Students supervision
- In charge of cell culture reagents (testing, inventory management)
Communication skills :
- Project reporting to the supervisor
- Presentation and discussion of data internally and externally (7 poster presentations at national and international meetings)
- Scientific documents redaction (1 published scientific article, 7 abstracts and 7 posters)
- Writing of 4 scientific projects for funds (4 grants obtained via Association pour la Recherche sur le Cancer, Fondation pour la Recherche Médicale, Conseil Régional de la Martinique, French Lowe Syndrome Association)
Scientific skills :
- Design and development of GLP experimental procedures
- Analyses and interpretation of results
- Team working and troubleshooting abilities
- Literature survey
Trainings :
- Scientific images edition with Photoshop software
  INSERM
  June 2006
- Fire training and handling of fire extinguishers
  Faculty of Medicine Paris Descartes
  May 2006
- Awareness of the prevention of occupational hazards in research laboratories
  INSERM
  November 2005
Publications :
The iodide transport NIS Regulates cell motility and invasiveness by interacting with the RhoGEF LARG cancer.
Claire Lacoste, Julie Herve Myriam Bou Nader, Moniaux Nicolas, Alexandre Dos Santos, Yannick Valogne, Rodrick Montjean, Dorseuil Olivier, Didier Samuel, Doris Cassio, Carla Portulano, Nancy Carrasco, Christian Bréchot & Jamila Faivre.
Cancer Res. 1 November 2012, 72 (21) :5505-15.
http://www.ncbi.nlm.nih.gov/pubmed/22962269
Poster communications:
Upregulation of the sodium iodide symporter in cholangiocarcinoma is associated with increased cell migration and invasion
C. Lacoste, J. Herve, N. Moniaux, F. Faitot, A. Dos Santos, R. Montjean, O. Dorseuil, D. Samuel, D. Cassio, C. Brechot, J. Faivre
American Association for the Study of Liver Diseases: Liver Meeting (Boston, USA. 29th oct- 2nd november 2010)
http://www.hepmag.com/conference/aasld2010.shtml

Implication of OCRL1-A, the Lowe syndrome protein, in trafficking and signaling in human neuronal cells
Michaël Trichet, Rodrick Montjean, Pierrette Desbois, Gérard Gacon, Olivier Dorseuil
Mental Retardation: from genes to synapses, functions and dysfunctions (Roscoff, France. 7th-11th November 2010).
http://www.anae-revue.org/article-conference-jacques-monod-sur-le-retard-mental---7-11-octobre-2010-57038516.html

Syndrome Oculo-Cérébro-Rénal de Lowe et protéine Ocrl1 : étude dans les cellules du système nerveux et du cristallin
Michaël Trichet, Rodrick Montjean, Pierrette Desbois, Gérard Gacon, Olivier Dorseuil.
11éme forum de l'école doctorale Gc2iD (Rueil-Malmaison, France. 20th-21th march 2008).

Rôle physiologique d'OCRL1, la protéine altérée dans le syndrome de Lowe, à la membrane plasmique de fibroblastes cutanés
Rodrick Montjean, Adèle Faucherre, Michaël Trichet, Pierrette Desbois, Gérard Gacon et Olivier Dorseuil
10éme forum de l'école doctorale Gc2iD (Rueil-Malmaison, France. 30th-31th march 2007).

Role of Ocrl1, the Lowe protein, in fibroblasts plasma membrane
Rodrick Montjean, Adèle Faucherre, Michaël Trichet, Pierrette Desbois, Gérard Gacon, Olivier Dorseuil.
Cell migration in development and disease (Paris, France. 20th-21th November 2006).

Etude des mécanismes moléculaires d’action d’Ocrl1 (PIP 5 phosphatase et RhoGAP) à la membrane plasmique des fibroblastes
Rodrick Montjean, Adèle Faucherre, Pierrette Desbois, Gérard Gacon, Olivier Dorseuil
Colloque des «petites protéines G» (Toulouse, France. May 2005).

Interaction de la protéine OCRL1 avec les GTPases Rho et conséquences fonctionnelles dans les cellules de patients Lowe
A. Faucherre, R. Montjean, P.Desbois, V. Satre , J. Lunardi , O. Dorseuil, G. Gacon
Colloque «GIS maladies rares» (30th-31th may 2005).
http://www.myobase.org/opac/index.php?lvl=notice_display&id=47473

Research topic:
Impact of PRA/PRB ratio modulation on apoptosis in breast cancer cell lines.

Education

Skills

International CRO supervision

Expert
Internal technical team management

Expert
National and International academic collaborators management

Expert
Team working

Expert
Design and development of experimental procedures and execution

Expert
Analyses and interpretation of results

Expert
Presentation and discussion of data in national and international meetings

Expert
Funding writing and obtention

Advanced
Scientific Technical Advise redaction for Health authorities

Good
Writing scientific documents (articles, abstracts, reports, SOPs, posters)

Expert
Troubleshooting abilities

Expert
Literature and technologic monitoring

Expert
Scientific projects and articles revewing

Good

Primary cell culture (human dermal fibroblasts, mouse embryonic fibroblasts, murine embryonic hippocampal neurons)

Expert
Cell lines culture : epithelial cells (cos, HeLa, HEK, HK-2, MDCK, IMCD, SRA 01/04, T47D), cells from human neuroblastoma SH-SY5Y

Expert
3-D cell culture, cell culture on Petri dishes, cell polarization on transwell filters.

Advanced
Cellular in vitro tests: Measurement of trans epithelial resistance, Migration assay (wound healing and transwell), Uptake assay (EGF receptor, albumin), Proliferation assay (BrDU), Adhesion assay (Vybrant), Calcium live-imaging

Expert
Cellular biology: Murine embryos extraction from uterine horns and isolation of embryonic hippocampi, transitory transfection in primary cells and in cell lines (liposome, electroporation), establishment of stable cell lines, production and viral infection

Expert
Molecular biology:
DNA and RNA extraction
PCR and qRT-PCR
Site-directed mutagenesis
Cloning and sub cloning
Bacteria transformation
Transcriptomic analyses

Advanced
Biochemistry:
Protein extraction, western blot and digital quantification
Production and purification of recombinant proteins
GST Pull-Down
Co-immunoprecipitation

Expert
Informatics: Images acquisition, treatment and analysis: MetaMorph (acquisition), ImageJ (treatment, analysis), Photoshop, Imaris (analysis).GraphPad (Prism), Endnote.Bio-informatics: databases (PubMed, Human protein atlas).

Expert
Cell imaging: Fixation and cellular staining (immunofluorescence), Wild-field and confocal microscopy, Videomicroscopy

Expert

BTS student (1)

Expert
ESTBA student (1)

Expert
EPHE student (1)

Expert
Master degree student (3)

Advanced
Technician (2)

Expert
Engineer (3)

Expert
Supervision of "Apprentis chercheurs" (2013-2014)
http://cochin.inserm.fr/enseignement/apprentis-chercheurs/les-apprentis-chercheurs-a-linstitut-cochin/pa_model2?set_language=en

Advanced

English : C1 level (CECRL)
German : notions

Interests

Play the Guitar (14 years at the Pierrefitte sur Seine Conservatoire)

West Indies (Martinique, Guadeloupe, Dominica)
America (United States of America, Canada)
Europe (Spain, Italy, Netherlands, Belgium, England, Germany, Switzerland, Poland, Greece)
Africa (Mali, Tunisia)

- Taekwondo tutor (black belt, 1st degree and 3-time French champion) and secretary at SDUS Taekwondo club
- Kickboxing and Muay-Thai at Le Thillay Kick boxing club

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R&D and preclinical development manager

Discovery project manager

R&D Project manager

Research associate

Research associate

Research associate

PhD fellow

DEA student

PhD in biochemistry, cellular and molecular biology

Paris Descartes University

Master’s degree in reproductive physiology

Pierre et Marie Curie University

Scientific project management and communication

Technical

Team management

Languages

Music

Travel

Sports