Expression in mammalian cells of a synthetic metabolic network of recombinant plant and bacterial proteins: – In silico design of recombinant plasmids – Synthesis of plasmids using Gibson cloning method – Extraction, purification, and sequencing of plasmids – Culture and co-transfection of mammalian cells
Study of function and localization in mammalian cells of recombinant proteins: – Epifluorescence and confocal cell microscopy – Metabolites detection using spectromertry and HPLC
Business and marketing strategies definition for a potential synthetic biology start-up: – Situational Analysis – Unique Selling Proposition – Marketing Strategies – Implementation Plan
