Laura Bencheikh
Research associate
34 years old
Driving License
Chevilly-Larue (94550) France
Professional Status
Unemployed
Available
About Me
5 years of hands-on experience in research laboratory (hematology, immunology and oncology). Extensive experience with primary blood cells sorting and culture, highly skilled in molecular biology, cell biology, biochemistry, imaging and flow cytometry. Looking for new challenges in biotechnology firms based in the United States.
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Primary blood cell sorting and banking
PBMC isolation (Ficoll) from whole blood, buffy coat, bone marrow, cord blood (over 250 samples processed). Granulocyte purification. Magnetic (AutoMacs) and FACS sorting of CD34+ cells, T cells, Monocytes, immature granulocytes (leukemia samples). Freezing/thawing of PBMC, CD34+, BMNC, T lymphocytes.Expert
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Cell line sterile culture and maintenance
Adherent and non adherent cell lines (293T, MDA-MB231, U937, THP-1, K562, UT7, BA/F3, HL-60, JAR, LCL, NKL, HeLa). Long-term culture (several months) with no contamination. Frozen stock maintenance, regular mycoplasm assessment.Expert
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Primary blood cell culture
Monocyte differentiation into macrophages and dendritic cells. CD34+ myeloid differentiation in liquid culture. T cell cutlure and proliferation assay.Advanced
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Molecular cloning
Cloned cDNA in a lentiviral expression vector, cloned RNA guides, promoter from genomic DNA, recruiting sites. Sub-cloning in TOPO vector. Cloning by recombination technique and restriction enzymes.Good
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CRISPR/Cas9
Designed and cloned guide RNA into a lentiviral vector. Transduction of cell line and sorting of positive cells (GFP+). Single cell cloning and expansion. Selection of the clones homozygous for the deletion by PCR.Good
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RNA and DNA extraction
With commercial kits and trizolAdvanced
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PCR/RT-qPCR
On genomic DNA, plasmids, cDNA generated by reverse transcription of RNA. Optimization of PCR conditions.Advanced
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Mutagenesis
Deletion of domains, of promoter region, insertion of tag or NLS sequences, point mutation (suppression of enzymatic activity)Expert
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Lentiviral vector production and transduction
30 productions with a pRRL backbone. Titration performed by flow cytometry (GFP).Good
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Transfection
Transfection of cell lines by lipofection and of primary monocytes by electroporationGood
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Sanger sequencing
On plasmids, purified PCR and genomic DNAGood
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Primer design
For PCR, mutagenesis, RT-qPCR, ChIP-qPCR, sequencingGood
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Competent bacteria production
Produced and stored two batches of thermosensitive STBL3Intermediate
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ChIP-qPCR/ChIP-seq
Developed ChIP-qPCR and ChIP-seq protocol for M-CSFR using activemotif kits. ChIP of histone marks and transcription factor EGR1Advanced
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4Peaks
For sequencing results analysisExpert
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DNAstrider
Sequence alignment, primer design, choice of restriction enzymesExpert
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Protein extraction
Total protein extraction, nuclear, membrane, cytoplasmic protein extractionAdvanced
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Western blot
Over 150 gels. Study of cell signaling, protein expression, localization, phosphorylation, dimerization, cleavageExpert
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Immunoprecipitation
Good
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Nanopro
Analysis of ERK phosphorylation in normal and leukemic monocytesIntermediate
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Flow cytometry
12 color panel design. Work on LSRII and FortessaAdvanced
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FACS DIVA
Advanced
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FlowJo
Advanced
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Flow imaging
Design and analysis of experiments (study of protein localization). Work on ImagestreamX MKIIGood
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IDEAS
Analysis of live imaging dataGood
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Luminex
Intermediate
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Immunofluorescence
Confocal microscopy (3D images, protein localization assessment, signal quantification, colocalization) on cultured adherent cells or non adherent cells (cytospin). Work on LEICA SPE/SP8Advanced
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Live cell imaging
Follow of fluorescent ligand internalization (movie) on spinning disk microscopeIntermediate
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ImageJ
Good
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French
Mother tongueExpert
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English
Good
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Microsoft Office
Excel (graphics, database, statistics), word, powerpoint (over 30 presentations)Advanced
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GraphPad Prism
Advanced
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Zotero
Bibliography for thesis, scientific article and internship reportsAdvanced
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Photoshop
Used for immunofluorescence image processingIntermediate
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Biostatistics
Notions
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Autonomy
Expert
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Team Work
Advanced
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Maintain detailed experimental records
Expert
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Communication (oral and written)
Expert
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Learn new skills or technologies
Advanced
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Data presentation
Advanced
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Organization
Expert
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Adaptability
Advanced
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Grant funding
Intermediate
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Supply and order management
Expert
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Student supervision
Advanced
