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Laura Bencheikh

Research associate

34 years old
Driving License
Chevilly-Larue (94550) France
Professional Status
Unemployed
Available
About Me
5 years of hands-on experience in research laboratory (hematology, immunology and oncology). Extensive experience with primary blood cells sorting and culture, highly skilled in molecular biology, cell biology, biochemistry, imaging and flow cytometry. Looking for new challenges in biotechnology firms based in the United States.
Resume created on DoYouBuzz
  • ChIP-seq protocol development in primary human blood cells
  • Establishment of knockout cell lines by CRISPR/Cas9 technique
  • Cell line sterile culture and maintenance
  • Cloning, mutagenesis, PCR optimization, siRNA assessment
  • Study of protein localization by confocal microscopy, immunofluorescence, live cell imaging, flow imaging
  • Blood cell magnetic sorting from blood, cord blood and bone marrow samples
  • Experiment design and execution, troubleshooting and interpretation of experiments
  • Antibody orders, cell culture room management, AutoMacs device routine care
  • Supervision of two master students
  • Scientific article writing
  • Grant funding
Detailed Description
  • Improved blood cell sorting efficiency by 50% and reduced costs by 100%
Company Description
First cancer research center in Europe
  • 12 color panel design for macrophage differentiation assessment by flow cytometry
  • Analyze routinely monocyte subsets in leukemia samples by flow cytometry
  • Human monocyte subsets isolation/culture development
  • Sample preparation for mass spectrometry
  • Analysis of leukemic monocyte signaling by Nanopro device
  • Analysis of M-CSFR signaling in monocyte subsets by Western Blot
Detailed Description
  • Flow cytometry study gave rise to a publication in blood journal : Selimoglu-Buet D, Wagner-Ballon O, Saada V, Bardet V, Itzykson R, Bencheikh L, et al.
    Characteristic repartition of monocyte subsets as a diagnostic signature of chronic myelomonocytic leukemia. Blood. 2015 Jun 4;125(23):3618-26.
Company Description
First european cancer research center
  • Blood cell sorting
  • Monocyte culture and differentiation into macrophage and dendritic cell
  • qPCR
  • Western Blot
Company Description
First european cancer research center
  • Development of HLA-G dosage protocol by Luminex and ELISA techniques
  • Student education
  • Flow cytometry
  • T cell culture
  • T cell proliferation assay
Company Description
French atomic energy commission with also a research activity in biology