Marine Lacroix
M.Sc. Eng., PhD student Immunology
Professional Status
Available soon
Available
About Me
I am a junior scientist with a 5-year experience in biotech industry in the field of autoimmune and neurological diseases and am now looking for challenging opportunities in translational biomedical research - Available in July 2015
- Results oriented, strong organization, prioritization, and time-management skills
- Proactive, autonomous
- Implementation of experimental design
- Data analysis and synthesis
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Writing skills
- Scientific communication: reports, posters for conferences
- OVC authorization writing
- Scientific communication: reports, posters for conferences
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Oral presentation
- National and international conferences
- Internal meetings with upper management
- Regular presentation of scientific articles – Journal Club
- National and international conferences
- Initiated to GMP/GLP/ICH-GCP compliance
- Initiated to QA environment e.g. SOP writing
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Functional assay implementation
- Proliferation (3H among others)
- Dye uptake
- Reporter gene assays
- Flow transmigration assay of monocytes or B cells to endothelial cells
- Electric cell-substrate impedance sensing
- Proliferation (3H among others)
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ELISA implementation
e.g. to titrate free/partially-bound antibody from pharmacokinetic study plasma samples (QCs; evaluation of interference, matrix effect)
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Multiplex assays
Luminex, evaluation of interference
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Flow cytometry on Calibur and Fortessa LSR II
- Design of pool of antibodies
- Whole blood, intracellular and phospho staining
- Immunophenotyping
- Design of pool of antibodies
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Molecular biology
- DNA/RNA extraction
- qRT-PCR
- Directed mutagenesis
- Cell transfection
- DNA/RNA extraction
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Cell culture
- Primary cells & cell lines
- PBMC isolation
- Cell immunomagnetic isolation
- Primary cells & cell lines
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Histology
- Organ fixation, dehydration, embedding, section
- Counterstainings
- Immunohistochemistry
- Immunofluorescence
- Organ fixation, dehydration, embedding, section
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In vivo preclinical experimentation
- Murine models of acute and chronic inflammation (CIA, EAE)
- Transgenic mice breeding & genotyping
- Murine models of acute and chronic inflammation (CIA, EAE)
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Monoclonal antibody production (small scale), purification & characterization
- Surface plasmon resonance
- Octet
- Western Blot
- N-glycan analysis by CGE-LIF
- Surface plasmon resonance
