Gautier ROBERT
Scientist in Drug Discovery
Scientist I within NIBR Autoimmunity/Transplantation
Novartis
Since January 2009
Basel
Switzerland
- Skills: primary cell culture, TLR antagonists assays, B cells/T cells isolation and characterization, FACS analysis, lentiviral infection, siRNA transfection, Western-Blot, real-time PCR, MSD technology, ELISA, AlphaLISA, Biacore, in-vivo work, radioactivity work, mentoring of trainees, slides preparation for project meetings, reports writing
Intern within NIBR Oncology department – Cellular Assay Unit
Novartis
March 2008
to November 2008
Basel
Switzerland
- "Identification and functional validation of a shRNA approach to efficiently down-regulate Tel-fusion proteins"
- Skills: siRNAs transfection, design and cloning of shRNAs, HIV-lentiviral vectors production and cells infection (BL2 level), Western-Blot, ELISA, nucleofection by Amaxa® system, real time PCR, FACS analysis
Intern within Genfit Company - Research and Development – Genomic area
Genfit
March 2007
to August 2007
Lille
France
- "Study of a G-protein coupled receptor (GPCR) in obesity"
- Skills: construction / cloning of chimeric GPCRs, coupling pathway identification
Trainee within the department
CNRS - IBL
July 2006
to August 2006
Lille
France
- "Development of one technique to titrate the Parvovirus H1 by qPCR"
- Skills: qPCR with Light Cycler technology, viral titration by dilutions in limit point
Trainee within a laboratory working on asthma
Centre Hospitalier Universitaire de Sherbrooke (Canada)
April 2005
to July 2005
Sherbrooke
Canada - Québec
- "Comparison of two DNA extraction methods to measure the telomere length"
- Skills: culture of fibroblasts, Southern Blot, calculation of the telomere length
Cellular and Molecular Engineering
Genomic and Proteomic - IUP Biotechnologies
Two-year university degree in technology
- Primary cells culture
- Cell-based assays development
- Compounds screening
- FACS analysis
- Lentivirus infection
- Cytokines detection (ELISA, MSD...)
- Isotopic labelling
- Real-time PCR
- Western-Blot
- siRNA transfection
- Cloning
- shRNA construction
- Licence for animal work
- Licence for radioactivity work
- Mentoring of trainees
- Pack Office
- GraphPad Prism
- Vector NTI
- Pharon, Helios, SpotFire (data visualisation)
