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Gautier ROBERT

Scientist in Drug Discovery

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Experiences
  • Skills: primary cell culture, TLR antagonists assays, B cells/T cells isolation and characterization, FACS analysis, lentiviral infection, siRNA transfection, Western-Blot, real-time PCR, MSD technology, ELISA, AlphaLISA, Biacore, in-vivo work, radioactivity work, mentoring of trainees, slides preparation for project meetings, reports writing
  • "Identification and functional validation of a shRNA approach to efficiently down-regulate Tel-fusion proteins"
  • Skills: siRNAs transfection, design and cloning of shRNAs, HIV-lentiviral vectors production and cells infection (BL2 level), Western-Blot, ELISA, nucleofection by Amaxa® system, real time PCR, FACS analysis
  • "Study of a G-protein coupled receptor (GPCR) in obesity"
  • Skills: construction / cloning of chimeric GPCRs, coupling pathway identification
  • "Development of one technique to titrate the Parvovirus H1 by qPCR"
  • Skills: qPCR with Light Cycler technology, viral titration by dilutions in limit point
  • "Comparison of two DNA extraction methods to measure the telomere length"
  • Skills: culture of fibroblasts, Southern Blot, calculation of the telomere length
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Resume created on DoYouBuzz