Skills — Estelle Hess

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Skills

Design and write research project
Research projects financed by (i) the French Ministère de l'Education, de la Recherche et de la technologie, (ii) "Ligue contre le cancer" association, (iii) European Research Council and (iv) the Walloon region.
Time management
Task planning and tracking of milestones
Communication of results
Scientific publications and reports writing and oral presentations of achievements
Scaning of scientific literature
Risk assessment
Data management, analyses and reporting
Design and conduct experiments
Supervision and training of Ph.D., M.Sc., technicians and students
Handle scientific collaboration

Development of diagnostic tests
Development of labratory (ELISA) and Point of Care teste (Laterafl Flow Assays)
Cellular biology
Cell culture (primary cells and cell lines), generation and production of monoclonal antibodies. Purification of immune and stromal cells (Ficoll, magnetic sorting), cell culture (cell lines and primary cells), ELISA, multicolor flow cytometry (FACSCalibur, FACSVerse, Cell Lab Quanta), transient and stable transfections, proliferation assays (3H-thymidine, MTS, CFSE), measurement of apoptosis (AnnexinV, Dioc6)
Microbiology
Culture of class 2 bacteria, habilitated to work in a BSL3 laboratory
Microscopy
Culture of class 2 bacteria, habilitated to work in a BSL3 laboratory
Molecular biology
DNA/RNA isolation, PCR techniques (PCR, RT-PCR and qRT-PCR), expression and purification of recombinant proteins in bacterial system, SDS-PAGE, Western-blotting
Animal experimentation
Animal experimentation and small animal surgery agreement (category C), strong experience with murine model

Estelle Hess