Estelle Hess

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Experiences

Identify targets and elucidate mechanisms of action of a drug candidate in the field of thromboinflammation with a focus on neutrophils
Responsible for the scientific oversight and design of research projects
Responsible for CRO selection, scientific and financial monitoring

Managed the R&D immunology team (5 people - PhD, MSc and technicians) for the development of new veterinary diagnostic kits
Provided customer support
Responsible for the cell culture facility (monoclonal antibody, virus and parasite production)

Developed veterinary diagnostic kits, including ELISA and point-of-care testing
Responsible for the cell culture facility (monoclonal antibody, virus and parasite production)

Studied the dog oral commensal Capnocytophaga canimorsus which causes rare but severe infections in humans
Wrote and carried out projects funded by the ERC and by the Walloon Region
Published 5 scientific papers (2 as a first author)
Scaning of scientific literature, writing scientific publications and patent
Training and supervision of students and technicians
Development of new protocols to study host-pathogen interactions
Redaction of ethic proposals for evaluation by animal experimentation committee

Unraveled new functions of the Receptor Activator of NF- κB Ligand in the control of adult lymph node plasticity and hair follicles cycle
Designing and conducting experiments
Management of different murine models
Scaning of scientific literature and writing scientific publications
Granting of two personal funding (3 years from the French Ministère de l’Education Nationale de la Recherche et de la Technologie and 1 year from the Ligue contre le cancer association)

Skills

Design and write research project

Research projects financed by (i) the French Ministère de l'Education, de la Recherche et de la technologie, (ii) "Ligue contre le cancer" association, (iii) European Research Council and (iv) the Walloon region.
Time management

Task planning and tracking of milestones
Communication of results

Scientific publications and reports writing and oral presentations of achievements
Scaning of scientific literature
Risk assessment
Data management, analyses and reporting
Design experiments
Supervision and training of Ph.D., M.Sc., technicians and students
Handle scientific collaboration

Development of diagnostic tests

Development of labratory (ELISA) and Point of Care teste (Laterafl Flow Assays)
Cellular biology

Cell culture (primary cells and cell lines), generation and production of monoclonal antibodies. Purification of immune and stromal cells (Ficoll, magnetic sorting), cell culture (cell lines and primary cells), ELISA, multicolor flow cytometry (FACSCalibur, FACSVerse, Cell Lab Quanta), transient and stable transfections, proliferation assays (3H-thymidine, MTS, CFSE), measurement of apoptosis (AnnexinV, Dioc6)
Microbiology

Culture of class 2 bacteria, habilitated to work in a BSL3 laboratory
Microscopy

Culture of class 2 bacteria, habilitated to work in a BSL3 laboratory
Molecular biology

DNA/RNA isolation, PCR techniques (PCR, RT-PCR and qRT-PCR), expression and purification of recombinant proteins in bacterial system, SDS-PAGE, Western-blotting
Animal experimentation

Animal experimentation and small animal surgery agreement (category C), strong experience with murine model

Experienced biologist and project manager