Cell culture (primary cells and cell lines), generation and production of monoclonal antibodies. Purification of immune and stromal cells (Ficoll, magnetic sorting), cell culture (cell lines and primary cells), ELISA, multicolor flow cytometry (FACSCalibur, FACSVerse, Cell Lab Quanta), transient and stable transfections, proliferation assays (3H-thymidine, MTS, CFSE), measurement of apoptosis (AnnexinV, Dioc6)