PhD subject : "Carbon sources management in bacteria Bacillus subtilis"
This project is model driven. We experimentally validated a regulation model of central carbon catabolism pathway of Bacillus subtilis. The model has been established first by mathematicians.
The validation is made by key enzyme purification and characterisation. Hypotesis suggested by the model are checked by Live Cell Array experiments.
KEY WORDS : Bacillus subtilis, glycolysis, neoglucogenesis, central carbon catabolism pathway, protein purification, enzymatics assays, Live Cell Array.
Detailed Description
SKILLS :
Microbiology, Biochemistry, Enzyme purification and characterization, Statistical analysis, Protocol creation and optimisation, Student supervision in an international lab (working, supervising, meeting in english)
ARTICLES :
Model-driven analysis of glyclolysis robustness of Bacillus subtilis, Charlotte Cousin, Anne Goelzer, Ivan Mijakovic, Vincent Fromion Metabolic engineering, submitted
Protein phosphorylation from the perspective of systems biology Derouiche A, Cousin C, Mijakovic I. Curr Opin Biotechnol. 2012 Aug;23(4):585-90. doi: 10.1016/j.copbio.2011.11.008. Epub 2011 Nov 24. Review. http://www.ncbi.nlm.nih.gov/pubmed/22119098
Protein-serine/threonine/tyrosine kinases in bacterial signaling and regulation. Cousin C, Derouiche A, Shi L, Pagot Y, Poncet S, Mijakovic I.FEMS Microbiol Lett. 2013 Jun 3. doi: 10.1111/1574-6968.12189. Review http://www.ncbi.nlm.nih.gov/pubmed/23731382
COMMUNICATION :
Talk : Model-driven strategy to perturb the robustness of glycolysis in Bacillus subtilis Charlotte Cousin, Anne Goelzer, Ivan Mijaković and Vincent Fromion 7th International Conference on Gram-positive microorganisms, Montecatini, Italy, June 2013
