Akshay Pujari
Biomedical Research, Computational Biology | Rubedo Life Sciences
32 years old
Driving License
San Francisco Bay Area United States (California)
Professional Status
Employed
Just looking around
About Me
Currently working at Rubedo Life Sciences primarily in the Bioinformatics / Computational Biology team. Previously, Research Associate II at an early staged drug discovery company – DiCE Molecules in Redwood City.
I graduated from the University of Massachusetts-Amherst with an MS in Mechanical, Biomedical Engineering. I worked as a Research Assistant in the Vascular Biology and Biofluids Laboratory, and did in-vivo, ex-vivo in-vitro and in-silico translational research work. With the diverse and cross-disciplinary nature of the research, my coursework included classes from mechanical and biomedical engineering, biology, animal science and computer science disciplines.
My background is in the research connecting the fields of Engineering, Biology, and Medicine, and my skillsets include but are not limited to: Biomedical Sciences, Computational Biology, Vascular Biology, Wet lab experimentation, Monolith Chemistry, Bioinformatics, Automation + Robotics and Engineering design. I have an extensive background in both life sciences and engineering domains. I am passionate about science and unification of disciplines to solve new problems relevant in understanding and improving human life.
I graduated from the University of Massachusetts-Amherst with an MS in Mechanical, Biomedical Engineering. I worked as a Research Assistant in the Vascular Biology and Biofluids Laboratory, and did in-vivo, ex-vivo in-vitro and in-silico translational research work. With the diverse and cross-disciplinary nature of the research, my coursework included classes from mechanical and biomedical engineering, biology, animal science and computer science disciplines.
My background is in the research connecting the fields of Engineering, Biology, and Medicine, and my skillsets include but are not limited to: Biomedical Sciences, Computational Biology, Vascular Biology, Wet lab experimentation, Monolith Chemistry, Bioinformatics, Automation + Robotics and Engineering design. I have an extensive background in both life sciences and engineering domains. I am passionate about science and unification of disciplines to solve new problems relevant in understanding and improving human life.
Graduate Research Assistant
Vascular Biology and Biofluids Laboratory, UMass Amherst
December 2015
to October 2017
Amherst
United States - Massachusetts
- Studied lymphatic fluid flow and lymphatic valves in-vivo in wild type and genetically modified mice.
- Studied effects of flow in cardiovascular & cerebral vessels; disease metastasis including cancer.
- Studied stenting, catheterization, medical devices, drugs and their effects to vasculature & disease.
- Explored several manuscripts to understand mechanism, development, effects, metastasis and clinical treatment of conditions such as atherosclerosis, thrombosis, aneurysms, myocardial infarctions, and lymphedema.
Detailed Description
- Performed intensive data analysis; generated algorithms for video, image processing, vessel edge detection and fluorescent particle tracking on in-vivo mice data. Used self-written custom MATLAB algorithms, Imaris, PTV Lab, and developed ImageJ Macros.
- Performed immunohistochemistry (IHC) experiments on mice tissues to demonstrate differential protein expressions on vascular endothelial cells subjected to differential flow pattern.
- Dissected mice samples; performed microscopy and developed in-vitro lab experiments/ protocols.
- Mentored two undergraduate students in the research group, presented journals in lab meetings.
- Presented my research on multiple platforms in both engineering and life sciences context. Presented poster in BMES Annual Meeting 2016, IALS Life Science Symposium 2016.
- Generated manuscripts, figures for publications. Efforts ongoing in the lab to finalize two manuscripts as first author through collaborative effort.
Company Description
The laboratory studies the interaction between fluid flow and biology, by integrating fluid dynamic engineering, cellular and molecular biology. Body fluids or biofluids, such as blood, lymph, and cerebrospinal fluid continuously interact with cells in the body eliciting biochemical and physical responses. The research seeks to elucidate the fluid flow characteristics and fluid flow-dependent biomolecular pathways relevant in medicine.
Company website
